​​​​​​We are seeking a self-motivated, curious and enthusiastic molecular biologist to join our High-throughput Screening Team to help us solve these and other interesting challenges in AAV engineering. You will work in a small-team setting to advance our discovery pipeline by measuring AAV capsid properties using existing methods and helping to develop new methods. In this role you will work closely with experienced scientists, with opportunities for mentorship, professional development, and cross-team collaboration.  

• Responsibilities: 

Maintain cultured mammalian cell lines following good aseptic technique

Perform transduction assays to evaluate virus infectivity

Extract, purify, and quantify nucleic acid from mammalian cells  

Prepare samples for next-generation sequencing

Assist in lab organization, including ordering supplies and restocking 

Collect, analyze and present assay results 

Follow established lab protocols and contribute to new experimental method development

Successfully collaborate with colleagues across teams

Identify experimental problems and actively engage in troubleshooting

• Basic qualifications:

PhD in biology, immunology, molecular biology, or related fields

2+ years hands-on wet lab experience

Experience in culturing mammalian cells and aseptic technique

Experience in DNA/RNA purification and analysis protocols (qPCR, RT-qPCR, etc.)

Molecular biology proficiency: PCR, gel electrophoresis and imaging, gel excision and purification 

Ability to follow advanced protocols and procedures

Ability to document and maintain an organized account of lab work and results

Skilled in Excel/Prism, Word, PowerPoint, and other relevant software

• Preferred qualifications:

3+ years of experience in biotech industry

Experienced in virus titration and infectivity assays (preferably AAV) e.g., potency assay, infectious center assay, Luciferase or GFP reporter assays 

Familiarity with antibody and immunological assays e.g., neutralization, ELISA, Luminex

Experience working with high-throughput assays, e.g., automated sample processing, multi-channel pipetting, molecular barcoding

Hands on molecular cloning experience

Experience in primary culture of mammalian cells or organoids

Cellular analysis: microscopy, staining, FACS, cell-sorting 

Experience in recombinant AAV production and purification